Comparative Studies Between Xanthomonas citri subsp. malvacearum Isolates, Causal Agent of the Bacterial Blight Disease of Cotton

Salah Eddin Khabbaz; D. Ladhalakshmi; Ahmed Abdelmagid

World Journal of Agricultural Research. 2017, 5(2), 64-72
DOI: 10.12691/WJAR-5-2-2

Original Research

Comparative Studies Between Xanthomonas citri subsp. malvacearum Isolates, Causal Agent of the Bacterial Blight Disease of Cotton

Salah Eddin Khabbaz^{1, 2,}, D. Ladhalakshmi³ and Ahmed Abdelmagid⁴

¹General Commission for Scientific Agricultural Research, Hama, Syria

²Current address of Salah Eddin Khabbaz: A&L Biologicals, 2136 Jetstream Road, London, ON, N5V 3P5, Canada

³Department of Plant Pathology, Directorate of Rice Research, Hyderabad, India

⁴Department of Plant Pathology, Assiut University, Assiut, Egypt

Pub. Date: February 23, 2017

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Cite this paper

Salah Eddin Khabbaz, D. Ladhalakshmi and Ahmed Abdelmagid. Comparative Studies Between Xanthomonas citri subsp. malvacearum Isolates, Causal Agent of the Bacterial Blight Disease of Cotton. World Journal of Agricultural Research. 2017; 5(2):64-72. doi: 10.12691/WJAR-5-2-2

Abstract

Out of 21 bacterial isolates collected during a survey from seven different cotton growing areas representing three districts of Tamil Nadu, India, only eight bacterial isolates of Xanthomonas axonopodis pv. malvacearum (Xam), now being called (Xanthomonas citri subsp. malvacearum) were found to be pathogenic to the susceptible cotton variety TCB-209. The eight pathogenic isolates were grouped into clusters A and B based on protein profiles. Cluster A had I₁, I₃, I₅, I₇ and I₈ isolates while cluster B had I₂, I₄ and I₆ isolates._. The isolates were morphologically similar, but varied in their cultural and biochemical characteristics. Isolate I₅ collected from Kavalur region showed a very high degree of virulence. Polyclonal antiserum was raised using the most virulent isolate I₅ and the titre was found to be 1:500. The antiserum also reacted positively with the other isolates. Pathogen was detected from different parts of the infected plant through DAC-ELISA and the leaves recorded maximum absorbance value indicating high level of bacterial infection.

Keywords

Bacterial blight, Cotton, DAC-ELISA, Xanthomonas axonopodis pv. malvacearum (X. citri subsp. malvacearum)

Copyright

This work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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