Distribution of Bioactive Compounds and Antioxidant Capacity of Different Parts of Justicia adhatoda L. (Acanthaceae)

M.M. Nipunika¹, D.C. Abeysinghe¹ and R.M. Dharmadasa^2,

¹Department of Plantation Management, Faculty of Agriculture and Plantation Management, Wayamba University of Sri Lanka, Makandura, Gonawila (NWP), 60170, Sri Lanka

²Industrial Technology Institute, 363, Bauddhaloka Mawatha, Colombo 07, 00700, Sri Lanka

Cite this paper

M.M. Nipunika, D.C. Abeysinghe and R.M. Dharmadasa. Distribution of Bioactive Compounds and Antioxidant Capacity of Different Parts of Justicia adhatoda L. (Acanthaceae). World Journal of Agricultural Research. 2022; 10(2):60-63. doi: 10.12691/WJAR-10-2-4

Abstract

Justicia adhatoda L is a well-known medicinal plant used in traditional systems of medicine globally. Different parts of J. adhatoda have been used for treatment of various ailments, mainly for the respiratory tract-based ailments. The present study was conducted to quantify the total antioxidant capacity (TAC), total phenolic content (TPC) and total flavonoid content (TFC) of different parts (mature leaves, immature leaves, flowers, bracts, soft stems, bark of mature stem and roots) of J. adhatoda using ferric reducing antioxidant power (FRAP) assay, modified Folin-Ciocalteu method and colorimetric method respectively. The results revealed that all tested parts of J. adhatoda contained marked amounts of TAC, TPC and TFC. Among tested parts, immature leaves showed a significantly higher TAC (19.28 ± 1.96 mg/TE g DW) followed by flowers (16.97 ± 0.99 mg/TE g DW) and bracts (14.19 ± 1.85 mg/TE g DW). Significantly the highest TPC (11.33 ± 0.14 mg/GAE g DW) and TFC (16.66 ± 3.06 mg/RE g DW) were observed in flowers followed by bracts and immature leaves. Moreover, there were positive correlations of TAC with TPC (R2 = 0.5411) and TFC (R2 = 0.5209). According to the results, it can be concluded that immature leaves, flowers and bracts of J. adhatoda contain marked amounts of bioactive compounds and hence could be effectively used for pharmaceutical industries.

Keywords

Acanthaceae, Justicia adhatoda, bioactive compounds, plant parts, total antioxidant capacity

Copyright

This work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

References

[1]	Hossain, M.T. and Hoq, M.O. (2016). Therapeutic use of Adhatoda vasica. Asian Journal of Medical and Biological Research, 2, (2), 156-163.
[2]	Rao, Bhaskar and Munjal, Minki and Patnayak, Amie and Loganathan, Karthik and Kumar, Gaurav 2013. Phytochemical Composition, Antioxidant, Antimicrobial and Cytotoxic Potential of Methanolic Extracts of Adhatoda vasica (Acanthaceae). Research J. Pharm. and Tech. 6. 997-1002.
[3]	Duraipandiyan, V., Al-Dhabi, N.A., Balachandran, C., Ignacimuthu, S., Sankar, C. and Balakrishna, K. (2015). Antimicrobial, antioxidant, and cytotoxic properties of vasicine acetate synthesized from vasicine isolated from Adhatoda vasica L. Biomed Res Int. 2015; 2015: 727304.
[4]	Gangwar, A.K. and Ghosh, A.K. (2014). Medicinal uses and pharmacological activity of Adhatoda vasica. International Journal of Herbal Medicine, 2, (1), 88-91.
[5]	Claeson, U.P., Malmfors, T., Wikman, G. and Bruhn, J.G. (2000). Adhatoda vasica: a critical review of ethnopharmacoylogical and toxicological data. Journal of Ethnopharmacology, 72, 1-20.
[6]	Chowdhury I, Rahman MA, Hashem MA, Bhuiyan MMH, Hajjar D, Alelwani W, Makki AA, Haque MA, Tangpong J, Bakhtiar MTB. Supplements of an aqueous combination of Justicia adhatoda and Ocimum tenuiflorum boost antioxidative effects and impede hyperlipidemia. Animal Model Exp Med. 2020 Apr 29; 3(2): 140-151.
[7]	Kumar, M., Kumar, A., Dandapat, S. and Sinha, M.P. (2013). Phytochemical screening and antioxidant potency of Adhatoda vasica and Vitex negundo. The Bioscan, 8, (2), 727-730.
[8]	Benzie, I.F. and Strain, J.J. (1996). The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Analytical Biochemistry, 239, (1), 70-76.
[9]	Abeysinghe, D.C., Li, X., Sun, C., Zhang, W., Zhou, C. and Chen, K. (2007). Bioactive compounds and antioxidant capacities in different edible tissues of citrus fruit of four species. Food Chemistry, 104, 1338-1344.
[10]	Liu, M., Li, X.Q., Weber, C., Lee, C.Y., Brown, J. and Liu, R.H. (2002). Antioxidant and antiproliferative activities of raspberries. Journal of Agricultural and Food Chemistry, 50, (10), 2926-2930.
[11]	Izzreen, N.Q. and Fadzelly, M.A.B. (2013). Phytochemicals and antioxidant properties of different parts of Camellia sinensis leaves from Sabah Tea Plantation in Sabah, Malaysia. International Food Research Journal, 20, (1), 307-312.
[12]	Panawala, P.B.C. Abeysinghe, D.C. and R.M. Dharmadasa, “Phytochemical Distribution and Bioactivity of Different Parts and Leaf Positions of Pimenta Dioica (L.) Merr (Myrtaceae).” World Journal of Agricultural Research, vol. 4, no. 5 (2016): 143-146.
[13]	Farhoosh, R., Golmovahhed, G.A. and Khodaparast, M.H.H. (2007). Antioxidant activity of various extracts of old tea leaves and black tea wastes (Camellia sinensis L.). Food Chemistry, 100, (1), 231-236.
[14]	Saha, J., Mukherjee, S., Gupta, K. and Gupta, B. (2013). High-performance thin-layer chromatographic analysis of antioxidants present in different parts of Saraca asoca (Roxb.) de Wilde. Journal of Pharmacy Research, 7(9), 798-803.
[15]	Kumar, M., Dandapat, S., Kumar, A. and Sinha, M.P. (2013). Pharmacological screening of leaf extract of Adhatoda vasica for therapeutic efficacy. Global Journal of Pharmacology, 8, (4), 494-500.
[16]	Pichersky, E. and Gang, D.R. (2000). Genetics and biochemistry of secondary metabolites in plants: an evolutionary perspective. Trends in Plant Science, 5, (10), 439-445.
[17]	Pagare, S., Bhatia, M., Tripathi, N., Pagare, S. and Bansal, Y.K. (2015). Secondary metabolites of plants and their role: Overview. Current Trends in Biotechnology and Pharmacy, 9, (3), 293-304.
[18]	Reddy, M.P., Shantha, T.R., Rao, V.R. and Venkateshwarlu, G. (2015). Pharmacognostical and Physicochemical Evaluation on the Flowers of Justicia adhatoda L. Research Journal of Pharmacognosy and Phytochemistry, 7, (2), 73-90.
[19]	Zhang, L., Ravipati, A.S., Koyyalamudi, S.R., Jeong, S.C., Reddy, N., Smith, P.T., Bartlett, J., Shanmugam, K., Münch, G. and Wu, M.J. (2011). Antioxidant and anti-inflammatory activities of selected medicinal plants containing phenolic and flavonoid compounds. Journal of Agricultural and Food Chemistry, 59, (23), 12361-12367.