Screening of Rhizobacterial Isolates for Biological Control of Fusarium Wilt Disease of Chickpea

Saifeldeen A. Abdalla¹, Ahmed M. El Naim^2, , Marmar A. El Siddig³, Adil Ali El Hussein³ and Elshiekh A. Ibrahim²

¹Department of Plant Protection, Faculty of Natural Resources and Environmental Studies, University of Kordofan, Elobied, Sudan

²Department of Crop Sciences, Faculty of Natural Resources and Environmental Studies, University of Kordofan, Elobied, Sudan

³Department of Botany, Faculty of Science, University of Khartoum, Sudan

Cite this paper

Saifeldeen A. Abdalla, Ahmed M. El Naim, Marmar A. El Siddig, Adil Ali El Hussein and Elshiekh A. Ibrahim. Screening of Rhizobacterial Isolates for Biological Control of Fusarium Wilt Disease of Chickpea. World Journal of Agricultural Research. 2022; 10(2):44-50. doi: 10.12691/WJAR-10-2-2

Abstract

The use of chemical control causing negative effects non-target environmental impacts and development of pesticide resistance to applied agent, The great interest in eco-friendly and sustainable agriculture, push towards gradually shifting to biological control instead of dependence on chemical. The Fusarium wilt is biotic stress that constraint the production and expansion of chickpea crop in Sudan. The aim of this study was to use rhizobacteria as bio control agent against chickpea Fusarium wilt. Eighteen soil samples taken from chickpea rhizosphere collected from six locations in central and Northern Sudan (three samples from each location). The chickpea rhizospheric bacteria were recovered from the 18 soil samples and their antagonistic activity against the most virulent FOC isolate was evaluated in vitro (using 76 rhizobacterial isolates) and in planta (using the ten most potential rhizobacterial isolates). 31 out of 76 isolates (nominated as SA1, SA2…., SA31) were considered as virulent bacterial isolates, shown clear inhibition zones against the most virulent FOC isolate (FOCS9). The widest inhibition zone diameter (25 mm) was recorded for isolate SA1 (No. 1) and the lowest zones (13.0 and 13.7 mm) were recorded for isolates SA30 (No. 30) and SA31 (no. 31), respectively. Generally, the in planta application of rhizobacterial isolates as biological control agents reduced the disease incidence compared with the controls.

Keywords

diseases incidence, Fusarium oxysporum, Cicer arietinum, antagonistic bacteria

Copyright

This work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

References

[1]	Nikam P.S, Jagtap G.P, Sontakke P.L (2011). Survey, Surveillance and Cultural Characteristics of Chickpea Wilt Caused by Fusarium oxysporum f.sp ciceris Afr.J.Agric.Res. 6(7):1913-1917.
[2]	Cerif M, Arfaoui A and Rhaiem A. (2007). Phenolic compounds and their role in bio-control of chickpea to fungal pathogenic attacks. Tunisian Journal Plant Protection 2: 7-21.
[3]	Jimenez-Gasco MM, Jimenes-Diaz RM (2003). Development a specific polymerase chain reaction-based assay for the identification of Fusarium oxysporum f.sp. ciceris and its pathogenic races 0, 1A, 5, and 6. Phytopathology 93:200-209.
[4]	Navas-Cortes JA, Hau B, Jimenes-Dias RM (1998) Effect of sowing date, host cultivar and race of Fusarium oxysporum f.sp ciceris on development of Fusarium wilt of chickpea. Phytopathology. 88:1338-1346.
[5]	Landa, B.B., Navas-Cortes, J.A and Jimenez-Diaz, R.M. (2004) Integrated management of Fusarium wilt of chickpea with sowing date, host resistance and biological control. Phytopatholgy, Vol. 94, PP. 946-960.
[6]	Gerhardson, B. (2002). Biological substitutes for pesticides. Trends in Biotechnology 20, 338-343.
[7]	Reddy, P.P (2014). Plant Growth Promoting Rhizobacteria for Horticulture Crop Protection. Springer India.
[8]	Schmidt CS, Agostini F, Leifert C, Killham K, Mullins CE (2004) Influence of soil temperature and matric potential on sugar beet seedling colonization and suppression of Pythium damping-off by the antagonistic bacteria Pueudomonas fluorescens and Bacillus subtilis phytopathology 94:351-363.
[9]	Intana W, Yenjit P,Suwanno T, Sattasakulchai S, Suwanno M, Chamswarng C (2008) Efficacy of antifungal metabolites Bacillus spp. For controlling tomato damping-off caused by Pythium aphanidermatum. Walailak Journal Science and Technology 5(1): 29-38.
[10]	Anjaiah, V.; Cornlis. P. and Kooedam, N. (2003). Effect genotype and root colonization in biological control of Fusrium wilts in pigeonpea and chickpea by Pseudomonas aeruginosa PNAI. Canadian Journal of Microbiology, Vol. 49, No. 2, 85-91.
[11]	]Hervas A, Landa BB, Jimenez-Diaz RM (1997) Influence of chickpea genotype and Bacillus sp. On protectin of Fusarium wilt by seed treatment with nonpathogenic Fusarium oxysporum. Eur J Plant Pathol 103:631-642.
[12]	Landa BB, Hervas A, Bettiol W, Jimenez-Diaz RM. (1997) Antagonistic activity of bacteria from the chickpea rhizosphere against Fusrium oxysporum f.sp. ciceris. Phytoparasitica 25(4): 305-318.
[13]	Nelson LM. (2004). PGPR: prospects of new inoculants. Okanagan University, Kelowna.
[14]	Saharan. B.S and Nehra. V. (2011). Plant Growth Promoting Rhizobacteria: A Critical Review. Life Science and Medicine Research 21: 1-30.
[15]	Abdalla, S.A, Algam, S.A.E, Ibrahim, E.A, El Naim, A.M. (2014) Invitro Screening of Bacillus Isolates for Biological Control of Early Blight Disease of Tomato in Shambat Soil. World Journal of Agricultural Research, Vol. 2, 47-50.
[16]	Pajand, N. and Paul, A.J., (2000). Endophytic Bacteria Induce Growth Promotion and Wilt Disease Suppression in Oilseed Rap and Tomato. Biological Control. 18: 208-215.
[17]	Algam, S.A.E. (2005) Biological control of bacterial wilt of tomato in green house environment by Bacillus spp. Isolated from tomato rhizosphere, Ph.D thesis, Zhejian University.
[18]	Kloepper. J.W (1993) Plant growth promoting rhizobacteria as biological control agents. In: Metting FB Jr (ed) Soil microbial ecology-applications in agricultural and environmental management. Marcel Dekker, New York, pp 255-274.
[19]	León, M 1, P M Yaryura, M S Montecchia, A I Hernández, O S Correa, N L Pucheu, N L Kerber, A F García (2009). Antifungal activity of selected indigenous pseudomonas and bacillus from the soybean rhizosphere. International Journal of Microbiology, 2009, 1-9.
[20]	Kumari, S., Khanna, V (2014). Effect of antagonistic Rhizobacteria coinoculated with Mesorhizobium ciceris on control of fusarium wilt in chickpea (Cicer arietinum L.). African Journal of Microbiology Research 8(12): 1255-1265.
[21]	Ahmed, N. and Adam,A. (2014). Evaluation of some chickpea cultivars for fusarium wilt disease in Sudan. Tropentag, “Bridging the gap between increasing knowelge and decreasing resources”. Pargue, Czech Republic. 6.14: 4. 438.