In vitro Propagation of Carica papaya L. Variety ‘Horana Papaya Hybrid 01’ Using Shoot Tip

S.M. Waidyaratne^1, , L.G.I. Samanmalie² and P.K.C. Buddhinie³

¹Department of Plant Sciences, University of Colombo, Colombo, 00300, Sri Lanka

²Plant Virus Indexing Centre, Department of Agriculture, Homagama, 10206, Sri Lanka

³Department of Botany, Faculty of Applied Sciences, University of Sri Jayewardenepura, Nugegoda, 10250, Sri Lanka

Cite this paper

S.M. Waidyaratne, L.G.I. Samanmalie and P.K.C. Buddhinie. In vitro Propagation of Carica papaya L. Variety ‘Horana Papaya Hybrid 01’ Using Shoot Tip. World Journal of Agricultural Research. 2022; 10(1):15-19. doi: 10.12691/WJAR-10-1-3

Abstract

In vitro plantlet regeneration ability of the local hybrid papaya variety Horana Papaya Hybrid 01 was evaluated using shoot tips from greenhouse-raised seedlings. Shoots tips surface sterilized in 20% Sodium Hypochlorite (Clorox^®) for 20 min were established in Murashige and Skoog (MS) basal medium containing 6-Benzylaminopurine (BAP; 0.0,0.5,1.0,1.5 mg/L) in combination with 1-Naphthalene Acetic Acid (NAA; 0.0,0.1,0.5 mg/L) for shoot multiplication. Considering the high mean number of shoots per explant (4.8 ± 0.5) and absence of calli, 1.0 mg/L BAP was the best treatment for direct organogenesis. Proliferated shoots were transferred to a 1.5 strength MS medium containing 0.25 mg/L BAP and Gibberellic Acid (GA₃; 0.0, 0.15, 0.30 mg/L) for further elongation. Elongated shootlets were placed in half-strength MS medium supplemented with Indole-3-Butyric Acid (IBA; 0.0,1.0, 2.0, 3.0 and 4.0 mg/L) for rooting. The highest root induction response (86%) and roots suitable for acclimatization were observed with 2.0 mg/L of IBA. Plantlets were acclimatized in poly cups containing a sterile potting mixture (soil, sand, compost, and coir dust; 1:1:1:1) and a survival rate of 75% was achieved under in vitro conditions. The findings of the present study can be optimized to develop a suitable in vitro micropropagation protocol for rapid clonal propagation of this papaya variety for producing true to type planting material.

Keywords

direct organogenesis, hybrid, micropropagation, papaya, shoot tip

Copyright

This work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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