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World Journal of Agricultural Research. 2020, 8(3), 75-83
DOI: 10.12691/WJAR-8-3-2
Original Research

Identification, Determination and Quantification of Indole-3-Acetic Acid Produced by Pseudomonas aeruginosa UPMP3 and Its Effect on The Growth of Oil Palm (Elaeis guineensis Jacq)

Waheeda Parvin1, 2, , Md. Mahbubur Rahman3, 2, Nisha T. Govender4 and Mui Yun Wong1, 5,

1Department of Plant Protection, Faculty of Agriculture. Universiti Putra Malaysia, Serdang, Malaysia

2Bangladesh Forest Research Institute, Chattogram, Bangladesh

3Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang, Malaysia

4Institute of Biology Systems (INBIOSIS), Universiti Kebangsaan Malaysia, Bangi, Malaysia

5Institute of Plantation Studies, Universiti Putra Malaysia, Serdang, Malaysia

Pub. Date: June 19, 2020

Cite this paper

Waheeda Parvin, Md. Mahbubur Rahman, Nisha T. Govender and Mui Yun Wong. Identification, Determination and Quantification of Indole-3-Acetic Acid Produced by Pseudomonas aeruginosa UPMP3 and Its Effect on The Growth of Oil Palm (Elaeis guineensis Jacq). World Journal of Agricultural Research. 2020; 8(3):75-83. doi: 10.12691/WJAR-8-3-2

Abstract

Pseudomonas species have founded as greatest and potentially most promising group of plant growth promoting rhizobacteria (PGPR). Pseudomonas aeruginosa UPMP3 is an important PGPR isolated from oil palm rhizosphere. This rhizobacteria is likely to synthesize and release phytohormone indole-3 acetic acid (IAA). Production of IAA is one of the main reasons to promote plant growth and yield. The aim of this study was to detect, identify and quantify the IAA production by P. aeruginosa UPMP3 in vitro and its influence on oil palm seedling growth. Nutrient broth medium supplemented with 1-5 mg/ml L-tryptophan and without L- tryptophan were used for bacterial culture. The pH levels of culture media were optimized under shaken and static conditions and incubated at 28±2°C in different incubation periods. The production of IAA by P. aeruginosa UPMP3 was extracted, purified, detected and quantified by Thin Layer Chromatography (TLC) and High Performance Liquid Chromatography (HPLC) analyses. Production of IAA was quantified by HPLC in liquid culture and achieved 12.08µg/ml with a retention time of 13.711 min. On the other hand, the maximum 52 µg/ml IAA was recorded in the medium supplemented with 4 mg/ml L- tryptophan in compare to control. The optimum pH level of the culture medium was recorded as 7 under shaken conditions at 150 rpm with 5 days incubation. The influence of IAA produced by the UPMP3 on oil palm seedling growth was carried out in the pot experiment. The germinated oil palm seedlings were treated with the extract of bacterial strain and observed a positive effect on seedling growth in respect to average root and leaf number, root, shoot, and leaf length compare to the synthetic IAA and the control.

Keywords

Indole-3-Acetic Acid, Pseudomonas aeruginosa UPMP3, Thin Layer Chromatography, High Performance Liquid Chromatography, Oil Palm

Copyright

Creative CommonsThis work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

References

[1]  El-Tarabily, K.A., “Promotion of tomato (Lycopersiconesculentum Mill.) plant growth by rhizosphere competent 1-aminocyclopropane-1-carboxylic acid deaminase-producing streptomycete actinomycetes”, Plant and Soil, 308: 161-174, 2008.
 
[2]  Ashrafuzzaman, M., Hossen, F.A., Ismail, M.R., Hoque, M.A., Islam, M.Z., Shahidullah, S.M. and Meon, S., “Efficiency of plant growth promoting Rhizobacteria (PGPR) for the enhancement of rice growth”, African Journal of Biotechnology, 8:1247-1252, 2009.
 
[3]  Compant, S. and Clement, C., “Plant growth-promoting bacteria in the rhizo- and endorhizosphere of plants: Their role, colonization, mechanisms involved and prospects for utilization”, Soil Biology & Biochemistry, 42: 669-678, 2010.
 
[4]  Patten, L. and Glick, R.B., “Bacterial biosynthesis of indole-3-acetic acid”, Canadian Journal of Microbiology, 42: 207–220, 1996.
 
[5]  Mastretta, C. and Barac, J., “Endophytic bacteria and their potential application to improve the phytoremediation of contaminated environments” Biotechnology & Genetic Engineering Reviews, 23: 175-297, 2006.
 
[6]  Khakipour, N., Khavazi, K., Mojallali, H., Pazira, E. and Asadirahmani, H., “Production of Auxin Hormone by Fluorescent Pseudomonads”, American-Eurasian Journal of Agricultural & Environmental Sciences, 4: 687-692, 2008.
 
[7]  Bhavdish, N., Johri, A., Sharma, J. and Virdi, S., “Rhizobacterial diversity in India and its influence on soil and plant health” Advances in Biochemical Engineering / Biotechnology, 84: 49-89, 2003.
 
[8]  Arkhipova, T.N., Veselov, S.U., Melentiev, A.I., Martynenko, E.V. and Kudoyarova, G.R., “Ability of bacterium Bacillus subtilis to produce cytokinins and to influence the growth and endogenous hormone content of lettuce plants”. Plant Soil, 272: 201-209, 2005.
 
[9]  Idris, E.E., Iglesias, E.J., Talon, M. and Borriss, R., “Tryptophan- dependent production of indole-3-acetic acid (IAA) affects level of plant growth promotion by Bacillus amyloliquefaciens FZB42”, Molecular Plant-Microbe Interactions, 20: 619-626, 2007.
 
[10]  Patten, C.H. and Glick, B.R., “Role of Pseudomonas putida Indole acetic Acid in development of the host plant root system” Applied and Environmental Microbiology, 68: 3795-3801, 2002.
 
[11]  Zaiton, S., Sariah, M. and Zainal Abidin, M.A., “Isolation and Characterization of Microbial Endophytes from Oil Palm Roots: Implication as Biocontrol Agents against Ganoderma”, The Planter, 82: 587-597, 2006.
 
[12]  Sambrook, J., Fritsch, E. F. and Maniatis, T., “Molecular cloning: a laboratory manual, 2nd ed.” Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y. 1989.
 
[13]  Sapak, Z., Sariah, M. and ZainalAbidin, M.A., “Effect of endophytic bacteria on growth and suppression of Ganoderma infection in oil palm”, International Journal of Agriculture and Biology, 10: 127-132, 2008.
 
[14]  Tien, T.M., Gaskins, M.H. and Hubbell, D.H., “Plant growth substances produced by Azospirillumbrasilense and their effect on the growth of pearl millet (Pennisetumamericanum L.)”, Applied and Environmental Microbiology, 37: 1016-1024, 1979.
 
[15]  Fried, B. and Sherma, J., “Thin-Layer Chromatography: Techniques and Applications”, Marcel Dekker, New York, 1982.
 
[16]  Ahmad, F., Ahmad, L. and Khan, M.S., “Indole Acetic Acid Production by the Indigenous Isolates of Azotobacter and Fluorescent Pseudomonas in the Presence and Absence of Tryptophan”, Turkish Journal of Biology, 29: 29-34, 2005.
 
[17]  Yasmin, S., Bakar, M. A. R., Malik, K. A. and Hafeez, F. Y., “Isolation, characterization and beneficial effects of rice associated plant growth promoting bacteria from Zanzibar soils”, Journal of Basic Microbiology, 44: 241-252, 2004.
 
[18]  Ritika, K.R., Ajay, K., Amit, K., Sandip, P., Amit, P. and Mohinder, K., “Indole Acetic Acid production by fluorescent Pseudomonas isolated from the rhizospheric soils of Malus and Pyrus”, Recent Research Science Technology, 4: 06-09, 2012.
 
[19]  María, G.T.-R., Sandra, A.V.-P., Jaime, B.-C., and Patricia, M.-N., “Isolation of Enterobacteria, Azotobacter sp. and Pseudomonas sp., Producers of Indole-3-Acetic acid and siderophores, from Colombian rice rhizosphere”, RevistaLatinoamericana de Microbiología, 42: 171-176, 2000.
 
[20]  Prashanth, S. and Mathivanan, N., “Growth promotion of groundnut by IAA producing rhizobacteria Bacillus licheniformis MML2501”, Archives of Phytopathology and Plant Protection, 43: 191-208, 2010.
 
[21]  Sharma, S., Verma, P. P. and Kaur, M., “Isolation, Purification and Estimation of IAA from Pseudomonas sp. using High-performance liquid Chromatography”, Journal of Pure and Applied Microbiology, 8(4): 1-6, 2014.
 
[22]  Crozier, A., Arruda, P., Jasmin, J.M., Monterio, A.M. and Sandberg, G., “Analysis of IAA and related indoles in culture medium from Azospirillumlipoferum and A. brasilense”, Applied and Environmental Microbiology, 54: 2833-2837, 1988.
 
[23]  Khalifah, R.A., Lowell, N., Lewis and Coogins, C.W., “Differentiation between indole acetic acid the Citrus auxin by column chromatography”, Plant Physiology, 41: 208-210, 1966.
 
[24]  Crozier, A. and Reeve, D.R., “The application of high performance liquid chromatography to the analysis of plant hormones. In (ed.) Plant growth regulation”, Proc. 4 Int. Conf. Plant Growth Substances, New York: P.E. Pilet, pp 67- 76, 1977.
 
[25]  Datta, C. and Basu, P.S., “Indole acetic acid production by a Rhizobium species from root nodules of a leguminous shrub, Cajanuscajan”, Microbiological Research, 155: 123-127, 2000.
 
[26]  Karnwal, A., “Production of Indole Acetic Acid by Fluorescent Pseudomonas in the Presence of L-Tryptophan and Rice Root Exudates” Journal of Plant Pathology, 91: 61-63, 2009.
 
[27]  Parvin, W., Jahan, Q. S. A., Rahman, M.M. and Wong, M.Y., “In vitro Screening and Optimization of IAA Production from Plant Growth Promoting Rhizobacteria Burkholderiacepacia UPMB3”, Plant Tissue Culture and Biotechnology, 28(1): 25-34, 2018.
 
[28]  Shirokikh, I.G., Zenova, G.M., Merzaeva, O.V., Lapygina, E.V., Batalova, G.A. and Lysak, L.V., “Actinomycetes in the prokaryotic complex of the rhizosphere of oats in a soddy-podzolic soil”, Eurasian Soil Science, 40: 158-162, 2007.
 
[29]  Yurekli, F., Geckil, H. and Topcuoglu, F., “The synthesis of indole-3-acetic acid by the industrially important white-rot fungus Lentinussajor-caju under different culture conditions”, Mycological Research, 107: 305-309, 2003.
 
[30]  Madhuri, M., “Screening of rhizobia for indole acetic acid production”, Annals of Biological Research, 2 (4): 460-468, 2011.
 
[31]  Sutthinan, K., Akira, Y., John, F.P. and Saisamorn, L., “Indole-3-acetic acid production by Streptomyces sp. isolated from some Thai medicinal plant rhizosphere soils”, EurAsian Journal of BioSciences, 4: 23-32, 2010.
 
[32]  Molina-Favero, C., Creus, C.M., Lanteri, M.L., Correa-Aragunde, N., Lombardo, M.C., Barassi, C.A. and Lamattina, L., “Nitric oxide and plant growth promoting rhizobacteria: Common features influencing root growth and development”, Advances in Botanical Research, 46: 1-33, 2007.