Variation of Phytochemical Content and Antioxidant Capacity of Domesticated and Non-Domesticated Momordica Charantia L. Populations in Different Maturity Stages

K.K.S. Withanage¹, D.C. Abeysinghe¹, R.M. Dharmadasa^2, , G.A. Prathapasinghe³ and L.J.A.P.A. Jayasooriya⁴

¹Department of Plantation Management, Faculty of Agriculture and Plantation Management, Wayamba University of Sri Lanka, Makandura, Gonawila (NWP), 60170, Sri Lanka

²Industrial Technology Institute, Bauddhaloka Mawatha, Colombo 07,00700, Sri Lanka

³Department of Livestock and Avian Sciences, Faculty of Livestock Fisheries and Nutrition, Wayamba University of Sri Lanka, Makandura, Gonawila (NWP), 60170, Sri Lanka

⁴Department of Basic Veterinary Science, Faculty of Veterinary Medicine and Animal Science, University of Peradeniya, Peradeniya, 20400, Sri Lanka

Cite this paper

K.K.S. Withanage, D.C. Abeysinghe, R.M. Dharmadasa, G.A. Prathapasinghe and L.J.A.P.A. Jayasooriya. Variation of Phytochemical Content and Antioxidant Capacity of Domesticated and Non-Domesticated Momordica Charantia L. Populations in Different Maturity Stages. World Journal of Agricultural Research. 2018; 6(4):140-143. doi: 10.12691/WJAR-6-4-4

Abstract

Momordica charantia Linn. commonly known as bitter melon or bitter gourd is an annual plant, belongs to family Cucurbitaceae. Bitter gourd possesses antidiabetic, anticancer, anti-inflammatory, antivirus, and cholesterol lowering effects. The content and composition of bioactive molecules are varied according to the plant parts and maturity levels of the plant. However, phytochemical distribution of leaves and fruits at different maturity stages of domesticated and non-domesticated populations of M. charantia populations cultivated in Sri Lanka is scattered or lacking. Therefore, the present study was undertaken to determine the phytochemical distribution of leaves and fruits of domesticated and non-domesticated populations of M. charantia at different maturity stages. Fruits were harvested at three different maturity stages viz. 10 days (immature), 20 days (mature) and 30 days (ripen) after fruit set. Total Antioxidant Capacity (TAC), Total Phenolic Content (TPC) and Total Flavonoid Content (TFC) were determined using Ferric Reducing Antioxidant Power (FRAP) assay, modified Folin-Ciocalteu colourimetric method and the colourimetric method respectively. Results revealed that TPC and TAC were higher in immature stages and decreased with the maturity. However, values were slightly increased at ripening stage. Significantly higher TPC, TFC and TAC were reported in leaves than fruits. In conclusion, since most of the tested phytochemicals were high in immature fruits and leaves of domesticated and non-domesticated populations of Momordica charantia, immature fruits and leaves can be recommended for the production of pharmaceuticals and nutraceuticals with elevated therapeutic activity.

Keywords

antioxidant capacity, Bitter gourd, flavonoids, Momordica charantia, phenolics

Copyright

This work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

References

[1]	Gupta, M., Sharma, S., Gautam, A.K., and Bhadauria, R. (2011). Momordica charantia Linn. (karela): nature’s silent healer. Pharmaceutical Science, 11, 32-36.
[2]	Goo, K.S., Ashari, S., Basuki, N. and Sugiharto, A.N. (2016). The bitter gourd Momordica charantia L.: morphological aspects, Charantin and vitamin C Contents. Journal of Agriculture and Veterinary Science, 9(10), 76-81.
[3]	Anon, (2006). Department of Agriculture. Available from: https://www.doa.gov.lk/ (Accessed on 20th August 2017).
[4]	Munsur, M.A.Z.A., Hague, M.S., Nasiruddin, K.M. and Hasan, M.J. (2007). Regeneration of bitter gourd (Mormodica charantia L) from leaf segments and root tips. Progressive Agriculture, 18(2), 1-9.
[5]	Behera, T.K., Singh, A.K., and Staub, J.E. (2008). Comparative analysis of genetic diversity in Indian bitter gourd (Mormodica charantia L.) using RAPD and ISSR markers for developing crop improvement strategies. Scientia Horticulture, 115(3), 209-217.
[6]	Daniel, P., Supe and Roymon, M.G. (2014). A review of the phytochemical analysis of Mormodica charantia. Advances in Pharmacy Biology, and Chemistry, 3(1), 2277-4688.
[7]	Abeysinghe, D.C., Li, X., Sun, C., Zhang, W., Zhou, C. and Chen, K. (2007). Bioactive compounds and antioxidant capacities in different edible tissues of citrus fruit of four species. Food Chemistry, 104, 1338-1344.
[8]	Liu, M., Li, X.Q., Weber, C., Lee, C.Y., Brown, J. and Liu, R.H. (2002). Antioxidant and anti-proliferative activities of raspberries. Agriculture and Food Chemistry, 50, 2926-2930.
[9]	Benzie, I.F. and Strain, J.J. (1996). The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power” the FRAP assay. Analytical Biochemistry, 239, 70-76.
[10]	Horax, R., Hettiarachchy, N., Kannan, A. and Chen, P. (2010). Proximate composition and amino acid and mineral contents of Momordica charantia L. pericarp and seeds at different maturity stages. Food Chemistry, 122, 1111-1115.
[11]	Serrano M., Guille’n F., Martinez-Romero D., Castillo S., Valero D. (2013). Chemical constituents and antioxidant activity of sweet cherry at different ripening stages, Agricultural Food Chemistry, 53, 2741-2745.
[12]	Siriamornpun, S. and Kaewseejan, N. (2017). Quality, bioactive compounds and antioxidant capacity of selected climacteric fruits with relation to their maturity.Scientia Horticulturae, 221, 33-42.
[13]	Kubola, K. and Siriamornpun, S. (2008). Phenolic contents and antioxidant activities of bitter gourd (Mormodica charantia L.) leaf, stem and fruit fraction extracts in vitro. Food Chemistry, 110, 881-890.
[14]	Zarei, M., Azizi, M. and Sadr, Z.B. (2010). Evaluation of physicochemical characteristics of pomegranate (Punica granatum L.) fruit during ripening. EDP Sciences.66, 121-12.
[15]	Olaniyi, A. and Opara,U.L. (2013).Changes in physical properties, chemical and elemental composition and antioxidant capacity of pomegranate (cv. Ruby) fruit at five maturity stages. Scientia Horticulture, 150, 37-46.
[16]	Gil, M.I., Tomas-Barberan F.A., Hess-Pierce B., Holcroft D.M. and Kader, A.A. (2000). Antioxidant activity of pomegranate juice and its relationship with phenolic composition and processing. Agricultural Food Chemistry, 48, 4581-4589.
[17]	Fischer, U.A., Carle, R. and Kammerer, D.R. (2011). Identification and quantification of phenolic compounds from pomegranate (Punica granatum L.) peel, mesocarp, aril and differently produced juices by HPLC-DAD-ESI/MSN. Food Chemistry, 127, 807-821.