Can Fucoidan Decrease the Mortalities Caused by Columnaris Disease in Nile Tilapia?

Hebatallah Ahmed Mahgoub^1,

¹Pathology Department, Faculty of Veterinary Medicine, Mansoura University, Egypt

Cite this paper

Hebatallah Ahmed Mahgoub. Can Fucoidan Decrease the Mortalities Caused by Columnaris Disease in Nile Tilapia?. World Journal of Agricultural Research. 2018; 6(1):1-4. doi: 10.12691/WJAR-6-1-1

Abstract

Columnaris disease is a serious disease in warm water fish. It is caused by Flavobacterium columnare, a Gram-negative bacterium. In this work, fucoidan was tested for its efficacy in decreasing the mortalities caused by Columnaris disease in Nile tilapia. Consequently, naturally-infected Nile tilapia with Flavobacterium columnare, showing eroded fins, rigid body figure, and ulcerated body surface, was treated with fucoidan (8 gm/kg ration) for 17 days. Flavobacterium columnare infection was confirmed by isolation on selective medium (cytophaga agar), from the skeletal muscle, tails fins, and gills, giving the typical rhizoid shape. It was also confirmed by Flavobacterium columnare specific PCR using selective primers for Flavobacterium columnare 16S ribosomal DNA. Fucoidan caused decreased the mortalities to nil and cured the eroded fins, the ulcerated body surface, and the rigid body figure. Fucoidan also decreased the tissue damage score to reach the normal histological score.

Keywords

columnaris disease, flavobacterium columnare, nile tilapia, fucoidan

Copyright

This work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

References

[1]	Seok, J., Kim, T.S., Kwon, H.J., Lee, S.P., Kang, M.H., Kim, B.J., et al. Efficacy of Cistanche Tubulosa and Laminaria Japonica Extracts (MK-R7) Supplement in Preventing Patterned Hair Loss and Promoting Scalp Health. Clin Nutr Res 2015; 4(2): 124-31.
[2]	Trejo-Avila, L.M., Morales-Martinez, M.E., Ricque-Marie, D., Cruz-Suarez, L.E., Zapata-Benavides, P., Moran-Santibanez, K., et al. In vitro anti-canine distemper virus activity of fucoidan extracted from the brown alga Cladosiphon okamuranus. Virusdisease 2014; 25(4): 474-80.
[3]	Pomin, V.H. Sulfated glycans in inflammation. Eur J Med Chem 2015; 92: 353-69.
[4]	Besednova, N.N., Zaporozhets, T.S., Somova, L.M., Kuznetsova, T.A. Review: prospects for the use of extracts and polysaccharides from marine algae to prevent and treat the diseases caused by Helicobacter pylori. Helicobacter 2015; 20(2):89-97.
[5]	Yu, S.H., Wu, S.J., Wu, J.Y., Wen, D.Y., Mi, F.L. Preparation of fucoidan-shelled and genipin-crosslinked chitosan beads for antibacterial application. Carbohydr Polym 2015; 126:97-107.
[6]	Aguilar-Briseno, J.A., Cruz-Suarez, L.E., Sassi, J.F., Ricque-Marie, D., Zapata-Benavides, P., Mendoza-Gamboa, E., et al. Sulphated polysaccharides from Ulva clathrata and Cladosiphon okamuranus seaweeds both inhibit viral attachment/entry and cell-cell fusion, in NDV infection. Mar Drugs 2015; 13(2):697-712.
[7]	Abuajah, C.I., Ogbonna, A.C., Osuji, C.M. Functional components and medicinal properties of food: a review. J Food Sci Technol 2015; 52(5): 2522-9.
[8]	Zhang, W., Oda, T., Yu, Q., Jin, J.O. Fucoidan from Macrocystis pyrifera has powerful immune-modulatory effects compared to three other fucoidans. Mar Drugs 2015; 13(3):1084-104.
[9]	Purnama, A., Aid-Launais, R., Haddad, O., Maire, M., Mantovani, D., Letourneur, D., et al. Fucoidan in a 3D scaffold interacts with vascular endothelial growth factor and promotes neovascularization in mice. Drug Deliv Transl Res 2015; 5(2): 187-97.
[10]	Park, J.H., Choi, S.H., Park, S.J., Lee, Y.J., Park, J.H., Song, P.H., et al. Promoting Wound Healing Using Low Molecular Weight Fucoidan in a Full-Thickness Dermal Excision Rat Model. Mar Drugs 2017; 15(4).
[11]	Sezer, A.D., Cevher, E., Hatipoglu, F., Ogurtan, Z., Bas, A.L., Akbuga, J. Preparation of fucoidan-chitosan hydrogel and its application as burn healing accelerator on rabbits. Biol Pharm Bull 2008; 31(12): 2326-33.
[12]	Tripathi, N.K., Latimer, K.S., Gregory, C.R., Ritchie, B.W., Wooley, R.E., Walker, R.L. Development and evaluation of an experimental model of cutaneous columnaris disease in koi Cyprinus carpio. J Vet Diagn Invest 2005; 17(1):45-54.
[13]	Amin, N.E., Abdallah, I.S., Faisal, M., Easa, M.e.-S., Alaway, T., Alyan, S.A. Columnaris infection among cultured Nile tilapia Oreochromis niloticus. Antonie Van Leeuwenhoek 1988; 54(6): 509-20.
[14]	Kubilay, A., Altun, S., Diler, O., Ekici, S. Isolation of Flavobacterium columnare from Cultured Rainbow Trout (Oncorhynchus mykiss) Fry in Turkey. Turkish Journal of Fisheries and Aquatic Sciences 2008; 8:165-9.
[15]	Bancroft, J.D., Gamble, M. Theory and practice of histological techniques. 6th ed. ed. [Edinburgh]: Churchill Livingstone, 2008.
[16]	Miles, A.A., Misra, S.S., Irwin, J.O. The estimation of the bactericidal power of the blood. J Hyg (Lond) 1938; 38(6):732-49.
[17]	Farmer, B. IMPROVED METHODS FOR THE ISOLATION AND CHARACTERIZATION OF FLAVOBACTERIUM COLUMNARE: Graduate Faculty of the Louisiana State University and Agricultural and Mechanical College; 2004.
[18]	Klopfleisch, R. Multiparametric and semiquantitative scoring systems for the evaluation of mouse model histopathology--a systematic review. BMC veterinary research 2013; 9:123.