Assessment of Maize (Zea mays L.) Exserohium Turcicum (Pass.) Leonard and Sugg. Isolates on Different Culture Media in Tanzania

Nwanosike M. R. O.^1, and Mabagala R. B.²

¹Department of Agricultural Education, Federal College of Education, Zaria, Nigeria

²Department of Crop Science and Production, Faculty of Agriculture, Sokoine University of Agriculture, Morogoro, Tanzania

Cite this paper

Nwanosike M. R. O. and Mabagala R. B.. Assessment of Maize (Zea mays L.) Exserohium Turcicum (Pass.) Leonard and Sugg. Isolates on Different Culture Media in Tanzania. World Journal of Agricultural Research. 2017; 5(6):292-298. doi: 10.12691/WJAR-5-6-2

Abstract

Morphological characteristics of twenty five isolates of E. turcicum collected from Kilimanjaro, Arusha, Morogoro, Iringa, Njombe and Mbeya Regions in Tanzania were studied in four solid media namely; V8 vegetable juice agar, malt extract agar, maize leaf extract agar and potato dextrose agar. The experiment was conducted twice and replicated three times (3 replicate × 25 isolates × 4 media) for each medium, making a total of 300 plates. The inoculated cultures were arranged in a Complete Randomized Design (CRD) and incubated at 25±1°C. The statistically significant differences (P ≤ 0.05) in colony growth, conidia germination, dry mycelial weight and rate of sporulation on the four solid media indicated the possibility of different strains of E. turcicum in the studied areas. However, colony growth was aggressive in V8 juice agar (5.7 cm) but conidia germination and rate of sporulation were high in malt extract agar. No isolate of E. turcicum germinated or sporulated on PDA. Isolates such as KHK₁₀, KHK₁₈, KHN₁₇, KHN₃, KMM₁₈ (Kilimanjaro Region), MMU₁₃ and MRI₄ (Mbeya Region), INM₈ (Iringa Region) and MMM₁₈ from Morogoro Region significantly yielded more colony growth, conidial germination, sporulation and dry mycelia compared to the other isolates. Molecular studies are needed to confirm the genetic variations amongst the isolates for sustainable maize breeding in Morogoro, Tanzania.

Keywords

Northern leaf blight, solid medium, Isolates, maize, Tanzania

Copyright

This work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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