World Journal of Agricultural Research. 2017, 5(5), 279-283
DOI: 10.12691/WJAR-5-5-5
Original Research

Evaluation of Stem Rust (Puccinia graminis f.sp tritici) Seedling Resistance in Kenyan Bread Wheat (Triticum aestivum L.) Mutant Lines

Emmaculate Akinyi Ogutu1, , Miriam Karwitha Charimbu1 and Peter Njoroge Njau2

1Egerton University, Department of Crops, Horticulture and Soils, Egerton, Kenya

2Highlands Agri-Consultant Limited, Njoro, Kenya

Pub. Date: October 23, 2017

Cite this paper

Emmaculate Akinyi Ogutu, Miriam Karwitha Charimbu and Peter Njoroge Njau. Evaluation of Stem Rust (Puccinia graminis f.sp tritici) Seedling Resistance in Kenyan Bread Wheat (Triticum aestivum L.) Mutant Lines. World Journal of Agricultural Research. 2017; 5(5):279-283. doi: 10.12691/WJAR-5-5-5


Race TTKSK (Ug99) of stem rust is a serious threat to wheat production worldwide because of its wide virulence on many cultivars and its rapid spread over countries. The objective of this study was to determine resistance to Puccinia graminis f.sp. tritici races of TTKTK and TTKSK at seedling stage among the wheat mutant lines. Duma, Kwale and NJBWII mutant lines were used since the parents are susceptible. Sixty three mutant lines along with six checks of NJBWII, Kwale, Duma, Cacuke, Robin and Kingbird were evaluated in the greenhouse at Kenya Agriculture and Livestock Organization, Njoro. A high frequency of mutant lines, 53.6% and 88.4% were resistant to TTKTK and TTKSK respectively, with low infection types ranging from “;” to “2+”. In addition, frequency of susceptibility was 46.38% for TTKTK and 11.59% for TTKSK on the evaluated genotypes. Resistance in these genotypes may be due to uncharacterized resistance genes or gene combinations that could not be resolved with the collection of races used. The information presented, when combined with the previous characterization of stem rust resistance genes will be useful for plant breeders in rationalizing germplasm enhancement programs.


stem rust, seedling stage resistance, mutation


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