Expression Analysis of the ADH Genes in Arabidopsis Plants Exposed to PEG-induced Water Stress

Thawda Myint; Sigit Ismawanto; Parameswari Namasivayam; Suhaimi Napis; Mohd Puad Abdulla

World Journal of Agricultural Research. 2015, 3(2), 57-65
DOI: 10.12691/WJAR-3-2-4

Original Research

Expression Analysis of the ADH Genes in Arabidopsis Plants Exposed to PEG-induced Water Stress

Thawda Myint^1,, Sigit Ismawanto¹, Parameswari Namasivayam¹, Suhaimi Napis¹ and Mohd Puad Abdulla¹

¹Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia

Pub. Date: March 20, 2015

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Thawda Myint, Sigit Ismawanto, Parameswari Namasivayam, Suhaimi Napis and Mohd Puad Abdulla. Expression Analysis of the ADH Genes in Arabidopsis Plants Exposed to PEG-induced Water Stress. World Journal of Agricultural Research. 2015; 3(2):57-65. doi: 10.12691/WJAR-3-2-4

Abstract

In plants, ethanolic fermentation occurs during limited oxygen condition and under certain environmental stresses. Many of the observations reported on the ADH1 gene during environmental stress conditions were obtained from studies that used single isoforms of the gene ADH1 even though many isoforms of the genes are known to be operational in plants based the complete genome sequence of more than 20 different plant species. Here, the Arabidopsis plants were exposed to polyethylene glycol-induced drought stress and the whole set of ADH (EC.1.1.1.1) genes as well as the enzyme activity of ADH in response to PEG-induced water stress condition was presented and discussed. At enzyme levels, both the root and leaf NADH-ADH activities were increased 5.9 and 4.4 folds when treated with 5% (w/v) PEG-20,000. At gene level, the majority of the ADH1 gene AT1G77120 and two of the ADH-like genes (AT1G64710 and AT5G24760) were up-regulated in the leaf and root. The result suggests that ADH1 together with other two more ADH-likes genes were responsive in the leaf and root operating along side during the PEG-induced water stress and these evidences support the conclusion that the capacity of ethanolic fermentation was enhanced in response to drought.

Keywords

Arabidopsis, PEG induced water stress, RWC, proline, chlorophyll, alcohol dehydrogenase

Copyright

This work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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