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World Journal of Agricultural Research. 2015, 3(1), 5-10
DOI: 10.12691/WJAR-3-1-2
Original Research

Screening of Kenyan Bread Wheat Varieties for Resistance to the Emerging Strains of Stem rust Fungi (Puccinia graminis f. sp. tritici) Race Ug99

Kimani N. C1, 2, , Onguso J.1 and Njau P.2

1Jomo Kenyatta University of Agriculture and Technology P.O Box 62000-00200 Nairobi, Kenya,

2Kenya Agricultural and Livestock Research Organisation, Food Crops Research Centre-Njoro P.O Box PRIVATE BAG-2107 Njoro, Kenya

Pub. Date: January 12, 2015

Cite this paper

Kimani N. C, Onguso J. and Njau P.. Screening of Kenyan Bread Wheat Varieties for Resistance to the Emerging Strains of Stem rust Fungi (Puccinia graminis f. sp. tritici) Race Ug99. World Journal of Agricultural Research. 2015; 3(1):5-10. doi: 10.12691/WJAR-3-1-2

Abstract

Stem rust disease of wheat caused by Puccinia graminis f. sp. tritici is of major concern because of its devastating effects on wheat. It can cause yield loss of up to 100% in susceptible varieties. East Africa has been designated as a “hot spot” of the stem rust pathogen as evidenced by the emergence of a new race of stem rust designated as TTKSK or better known as Ug99 and several of its variants. This pathogen therefore poses a threat to wheat production and hence to food security in Kenya. The frequent use of fungicides to control the disease also poses a potential adverse effect on the environment. The objective of this study was to screen a core collection of Kenyan bread wheat varieties to determine those with natural resistance to stem rust disease hence reduce the risk posed to food security and the environment. Twenty Kenyan commercial bread wheat varieties were screened for stem rust resistance under artificial disease epidemic simulation in the International Stem Rust Screening Field at Kenya Agricultural and Livestock Research Organisation, Food Crops Research Centre-Njoro, Kenya. The disease notes were taken using the Modified Cobb’s Scale and the Area Under Disease Progress Curve (AUDPC) values computed. Thirteen random samples of stem rust fungi were collected from the trial plot and analyzed using Ug99 race group-specific Single Nucleotide Polymorphism (SNP) markers. The varieties fell in three disease categories of resistant, intermediate and susceptible, with the most susceptible being Pasa and Kenya Swara being the most resistant. The mean AUDPC computed showed that there was variation in the AUDPC values among the varieties with the variety K.Swara having the lowest AUDPC value of 78.33 and variety Pasa having the highest value of 478.67. Analysis of Variance (ANOVA) showed that both AUDPC and disease scores had significant variation (P<0.0001) among the varieties. From the analysis of stem rust fungi samples two genotypes of stem rust race TTKSK (AF-001ad and AF-001aa) were detected indicating mutations within the same race variant. In conclusion there are Ug99 resistant Kenyan bread wheat varieties which hold a promise for food security. There is also evidence of further mutation within the TTKSK race variant and hence a possible increased virulence on the wheat genotypes.

Keywords

Stem rust, AUDPC of stem rust, Puccinia graminis f. sp. tritici, Emerging strains of Race Ug99, SNP markers

Copyright

Creative CommonsThis work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

References

[1]  Dyck P.L. and Kerber E.R., “Resistance of the race-specific type.” In: A.P. Roelfs & W.R. Bushnell (Eds). ‘The Cereal Rusts, Vol II’ pp 469-500. Academic Press, London. 1985.
 
[2]  Flor H.H., “Inheritance of reaction to rust in flax.” J. Agric. Res. 74: 241-262. 1947.
 
[3]  Studies of durable adult-plant resistances in 'Hope' and related cultivars to wheat rusts. Zeitschrift fur Pflanzenzuchtung 83: 350-367.
 
[4]  Johnson R. “Past, present and future opportunities in breeding for disease resistance, with examples from wheat.” Euphytica 63: pp 3-22. 1992.
 
[5]  Jin Y, Szabo L.J, Pretorius Z.A, Singh R.P, Ward R and Fetch T jr., “Detection of virulence to Resistance Gene within the Race TTKS of Puccinia graminins fsp tritici”, Plant Dis. 92: 923-926. 2008.
 
[6]  Kenya Institute for Public Policy Research and Analysis (KIPPRA), “Kenya Economic Report 2013, Creating an Enabling Environment for Stimulating Investment for Competitiveand Sustainable Counties” Nairobi, Kenya p 74. 2013.
 
[7]  Kota, R., Spielmeyer, W., McIntosh, R. A., and Lagudah, E. S., “Fine genetic mapping fails to dissociate durable stem rust resistance gene Sr2 from pseudo-black chaff in common wheat (Triticum aestivum L.)”. Theor. Appl. Genet. 112:492-499. 2005.
 
[8]  Mahbubjon Rahmatov. “Introductory Paper at the Faculty of Landscape Planning, Horticulture and Agricultural Science Swedish University of Agricultural Sciences 2013: 3 Alnarp, December, 2013.
 
[9]  Njau P.N., R. Wanyera, G. K. Macharia, J. Macharia, R. Singh and B. Keller, “Resistance in Kenyan bread wheat to recent eastern African isolate of stem rust, Puccinia graminis f. sp. tritici, Ug99” Journal of Plant Breeding and Crop Science, 1 (2): pp. 022-027. 2009.
 
[10]  Peterson, R. F., Champbell, A. B., and Hannah, A. E., “A diagramatic scale for estimating rust intensity of leaves and stem of cereals”. Peterson, R.F. C26: 496-500. 1948.
 
[11]  Pretorius Z.A, Singh RP, Wagoire W.W, Payne T.S., “Detection of virulence to wheat stem rust resistance gene Sr31 in Puccinia graminis f. sp. tritici in Uganda”. Plant Diseases; 84: 203. 2000.
 
[12]  Pretorius Z.A, Szabo L. J., Boshoff W. H. P., Herselman L., Visser B., “First report of a new TTKSF race of wheat stem rust (Puccinia graminis f. sp. Tritici) in South Africa and Zimbabwe.” Plant Diseases; 96: 590. 2012.
 
[13]  Roelfs AP, Martens JW., “An international system of nomenclature for Puccinia graminis f.sp. tritici.” Phytopathology; 78:526-33. 1988.
 
[14]  Singh Ravi P, Hodson David P, Huerta-Espino Julio, Jin Yuen, Bhavani Sridhar; Njau Peter; Herrera-Foessel Sybil, Singh Pawan K, Singh Sukhwinder, Velu Govindan, “The Emmergence of Ug99 Races of the Stem Rust Fungus is a Threat to World Wheat Production.”, Annu. Rev. Phytopathol 49: 465-481. 2011.
 
[15]  Singh R.P, D.P. Hodson, J. Huerta-Espino, Y. Jin, P. Njau, R. Wanyera, S.A. Herrera-Foessel, S. Bhavani, D. Singh and P.K. Singh (2008). “Global Status of Ug99 Spread and Efforts to Mitigate the Threat”, In: Preceding of International Conference on Wheat Stem Rust Ug99-A Threat to Food Security; (Eds.), GP Singh, K V Prabhu and Anju M Singh, Indian Agricultural Research Institute, New Delhi, India pp 85.
 
[16]  Sunderwirth, S. D., and Roelfs, A. P., “Greenhouse characterization of the adult plant resistance of Sr2 to wheat stem rust”. Phytopathology 70, 634-637. 1980.
 
[17]  Szabo, L.J., Crouch, J., “Development of a molecular assay system for the rapid detection and identification of Ug99 and related races of Puccinia graminis”. Meeting Abstract. p. 238, 2012.
 
[18]  United States Department of Agriculture (USDA) “Kenya Corn, Wheat and Rice Grain and Feed Annual Report” 2013.
 
[19]  Wanyera R, Kinyua MG, Jin Y, Singh RP., “The spread of stem rust caused by Puccinia graminis f. sp. tritici, with virulence on Sr31 in wheat in Eastern Africa.” Plant Diseases; 90: 113. 2006.
 
[20]  Wilcoxson, R.D., Skovmand, B. and Atif, A.H., “Evaluation of wheat cultivars ability to retard development of stem rust.” Annals of Applied Biology 80: 275-2181. 1975.