Genetics of Reniform Nematode Resistance in Gossypium arboreum Germplasm Line PI 529728

John E. Erpelding^1, and Salliana R. Stetina¹

¹USDA ARS Crop Genetics Research Unit, Stoneville, MS, United States

Cite this paper

John E. Erpelding and Salliana R. Stetina. Genetics of Reniform Nematode Resistance in Gossypium arboreum Germplasm Line PI 529728. World Journal of Agricultural Research. 2013; 1(4):48-53. doi: 10.12691/WJAR-1-4-1

Abstract

Reniform nematode (Rotylenchulus reniformis) is a serious pathogen of cotton in the United States and management has been difficult due to the lack of resistant upland cotton (Gossypium hirsutum) varieties. The diploid G. arboreum germplasm line PI 529728 was identified as a potential new source of R. reniformis resistance. Line PI 529728 was crossed with the highly susceptible G. arboreum germplasm line PI 529729 to develop F₁, BC₁F₁, and F₂ populations that were screened for nematode resistance under controlled environmental conditions to determine the genetics of resistance. The 10 F₁, 69 BC₁F₁, and 332 F₂ plants were inoculated with 1,000 vermiform nematodes and the number of swollen females on the root systems was determined 28 days after inoculation. The F₁ plants supported more nematodes than the susceptible control genotype PI 529251 (G. hirsutum accession Deltapine 16) indicating resistance was a recessive trait. For the BC₁F₁ and F₂ populations, plants supporting the same or a reduced level of infection that developed on the resistant control genotype PI 163068 (G. barbadense accession Texas 110) were rated as resistant. Based on this classification of resistance and susceptibility, it was predicted that a single recessive gene was conferring resistance; although, the BC₁F₁ population had more susceptible plants than expected. Additionally, highly resistant plants were observed in the BC₁F₁ and F₂ populations. This information will aid in the introgression of R. reniformis resistance from PI 529728 into upland cotton for the development of resistant varieties.

Keywords

cotton, germplasm, Gossypium arboreum, reniform nematode, resistance

Copyright

This work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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